project-name/
├── README.md                 # Project overview and getting started
├── .gitignore               # Exclude data, outputs, env files
├── environment.yml          # Conda environment (or requirements.txt)
├── data/                    # Input data (often gitignored)
│   ├── raw/                # Original, immutable data
│   ├── processed/          # Cleaned, transformed data
│   └── external/           # Third-party data
├── notebooks/               # Jupyter notebooks for exploration
│   ├── 01-exploration.ipynb
│   ├── 02-analysis.ipynb
│   └── figures/            # Notebook-generated figures
├── src/                     # Source code (reusable modules)
│   ├── __init__.py
│   ├── data_processing.py
│   ├── analysis.py
│   └── visualization.py
├── scripts/                 # Standalone scripts and workflows
│   ├── download_data.sh
│   └── run_pipeline.py
├── tests/                   # Unit tests
│   └── test_analysis.py
├── docs/                    # Documentation
│   ├── methods.md
│   └── references.md
├── results/                 # Analysis outputs (gitignored)
│   ├── figures/
│   ├── tables/
│   └── models/
└── config/                  # Configuration files
    └── analysis_config.yaml

project-name/
├── README.md
├── .gitignore
├── setup.py                 # Package configuration
├── requirements.txt         # or pyproject.toml
├── src/
│   └── package_name/
│       ├── __init__.py
│       ├── core.py
│       └── utils.py
├── tests/
│   ├── test_core.py
│   └── test_utils.py
├── docs/
│   ├── api.md
│   └── usage.md
├── examples/                # Example usage
│   └── example_workflow.py
└── .github/                 # CI/CD workflows
    └── workflows/
        └── tests.yml

project-name/
├── README.md
├── data/
│   ├── raw/                # Raw sequencing data
│   ├── reference/          # Reference genomes, annotations
│   └── processed/          # Workflow outputs
├── workflows/               # Galaxy .ga or Snakemake files
│   ├── preprocessing.ga
│   └── assembly.ga
├── config/
│   ├── workflow_params.yaml
│   └── sample_sheet.tsv
├── scripts/                # Helper scripts
│   ├── submit_workflow.py
│   └── quality_check.py
├── results/                # Final outputs
│   ├── figures/
│   ├── tables/
│   └── reports/
└── logs/                   # Workflow execution logs

notebooks/
├── 01-data-exploration.ipynb
├── 02-quality-control.ipynb
├── 03-statistical-analysis.ipynb
└── 04-visualization.ipynb

data/raw/
├── sample-A_hifi_reads_2026-01-15.fastq.gz
├── sample-B_hifi_reads_2026-01-15.fastq.gz
└── reference_genome_v3.fasta

# Python
__pycache__/
*.py[cod]
*$py.class
.venv/
venv/
*.egg-info/

# Jupyter
.ipynb_checkpoints/
*.ipynb_checkpoints

# Data
data/raw/
data/processed/
*.fastq.gz
*.bam
*.vcf.gz

# Outputs
results/
outputs/
*.png
*.pdf
*.html

# Logs
logs/
*.log

# Environment
.env
environment.local.yml

# OS
.DS_Store
Thumbs.db

data/
├── raw/                    # Original, immutable
├── interim/                # Intermediate processing steps
├── processed/              # Final, analysis-ready data
└── external/               # Third-party data

# Project Name

Brief description

## Installation

How to set up the environment

## Usage

How to run the analysis/code

## Project Structure

Brief overview of directories

## Data

Where data lives and how to access it

## Results

Where to find outputs

project/
├── script1.py
├── script2.py
├── data.csv
├── output1.png
├── output2.png
└── final_really_final_v3.xlsx

notebooks/
├── notebook1.ipynb
├── test.ipynb
├── analysis.ipynb
└── analysis_new.ipynb

project/
├── src/
│   ├── analysis.py
│   ├── data.csv          # Data in source code directory
│   └── figure1.png       # Output in source code directory

# Create standard research project structure
mkdir -p data/{raw,processed,external} notebooks scripts src tests docs results config
touch README.md .gitignore environment.yml